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 From
a practical point of view, there is no difference between separation of
microbial metabolites versus plant metabolites. Various countercurrent
instruments have been used in research in pharmaceutical companies for the
isolation of microbially produced metabolites, in particular antibiotics
(e.g. Chen, R.H., Hochlowski, J. E., McAlpine, J.B. and Rasmussen, R. R.
“Separation and purification of macrolides using the Ito multiplayer
horizontal coil planent centrifuge” J. Liq. Chromatog. 11, 191-201 (1988);
Martin, D. G., Peltonen, R. E. and Nielsen, J. W., “Preparative resolution
of an actinomycin complex by countercurrent chromatography in the coil
planet centrifuge”. J. Antibiotics, 39, 721-723 (1986); Brill, G. M.,
McAlpine, J. B., and Whittern, D. “Tirandalydigin, a novel tetramic acid
of the tirandamycin-streptolydigin type, II. Isolation and structural
characterization. J. Antibiotics, 41, 36-44 (1988); Onju, Y., Aoki, Y.,
Yamazoe, Y., Doki, Y. and Moriyama, T., “Isolation of nivalenol and
fusarenon-X from pressed bearley culture by centrifugal partition
chromatography”. J. Liq. Chromatog. 11, 2537-2546 (1988).
J. Glinski, using Sanki CPC model
LLN, equipped in preparative cartridges purified preparative quantities of
several biologically potent metabolites, including Cytochalasin C,
Rapamycin, and FK-506 (active component in the immunosuppressive drugs
Prograf and Tacrolimus. The same Sanki CPC was used for isolation of
avermectins from different batches of commercial avermectin.
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